The group, which KSI is part of, came up with the idea for the montage themselves.
As well as YouTube stars, footballer Rio Ferdinand, singer Jess Glynne, and Love Island narrator Iain Stirling are among well-known faces to appear.
The group says any advertising revenue earned from the video will be "donated to the NHS".
The pledge follows fitness instructor Joe Wicks' promise to give "every single penny" raised from his livestreamed YouTube PE sessions to the health service.
However, some people have expressed concern at the idea of the NHS being treated like a charity.
Lovely gesture as it is, part of me is a bit uncomfortable with this. The NHS isn’t a charity. Isn’t normalizing that idea a bit weird? Doesn’t it undermine the idea national insurance? And begin to eradicate politicians’ responsibilities to it? https://t.co/2MF3aQUePv
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