Enner Valencia scored his third goal of the World Cup before being taken off on a stretcher as Ecuador drew with the Netherlands to leave Group A wide open.
PSV Eindhoven forward Cody Gakpo had given the Netherlands the lead after just five minutes and four seconds - the quickest goal so far in Qatar - when he struck a shot beautifully into the top of the net.
But the Dutch struggled to build on their lead, with Ecuador's Pervis Estupinan having a goal disallowed on the stroke of half-time.
Ecuador fans were bouncing with excitement throughout the second half at the Khalifa International Stadium as they equalised just after the break and continued to create chances against a rattled Netherlands side.
The entire Ecuador squad ran over to the corner flag to celebrate with Valencia after he poked in the rebound from Estupinan's parried shot in the 49th minute - and Gonzalo Plata hit the crossbar with a long-range effort shortly after.
Captain Valencia, who scored both goals in Ecuador's victory over hosts Qatar on the opening night of the World Cup, was a constant threat but his evening was cut short due to injury.
The former West Ham and Everton striker went down in the final minute of normal time and was taken off on a stretcher, but was seen hobbling to his seat in the dugout.
The result means three teams from Group A will be battling it out for a place in the last 16 in the final group match - the Netherlands, Ecuador and Senegal - with all three capable of going through as table leaders.
With the Netherlands heavy favourites going into their final group game against bottom side Qatar, all eyes will be on a showdown between Ecuador and Senegal, which will dictate the table order.
"If you want to become world champion then of course you need to improve a lot as some of the opponents we are yet to face is playing at a much higher level," said Netherlands boss Louis van Gaal.
"We simply need to improve our game in terms of ball possession and we are working on that. Whether we'll make it in this tournament I can't guarantee that. But I think so."
Gakpo's opener was the first goal Ecuador had conceded in 701 minutes across all competitions - and a first since March - but the Netherlands struggled to create much as the game wore on.
Estupinan was left frustrated at half-time, with several of his team-mates remaining on the pitch to chat to the officials after his goal was ruled out when team-mate Jackson Porozo was offside and obstructing the view of the goalkeeper.
Van Gaal brought on Memphis Depay at half-time - a planned move as the Barcelona forward continues his return to fitness following injury - but it made little difference as Ecuador were unlucky not to pick up all three points.
Ecuador leader steps up again but at what cost?
The celebrations at full-time were subdued from Ecuador fans after a deserved point against strong opposition.
Valencia, who had an aura about him all evening and had the crowd on their feet on numerous occasions, has scored all three of Ecuador's goals so far at the tournament and they will need him on Tuesday for their crucial group decider with Senegal.
His injury in the final moments of the game will cause concern for Ecuador boss Gustavo Alfaro but otherwise it was a good evening for his side.
"[Valencia] is a top-level player when he wears the Ecuador jersey. I tell him that he will be successful in the World Cup even if he wasn't so much in the qualification campaign," said Alfaro.
"He is going through such a good spell. He is the captain."
Ecuador will be frustrated not to come away with more having had 14 shots in total - including four on target - and limiting the Netherlands to just the one, which was Gakpo's early opener.
Gakpo was on the scoresheet again having scored in the victory over Senegal, only enhancing his reputation in Qatar, but he failed to have an impact on the game afterwards.
The Netherlands have looked ropey in both of their group matches so far but are still in with a strong chance of progressing as group winners.
Player of the match
EstupiñánPervis Estupiñán
Netherlands
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Squad number8Player nameGakpo
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Squad number14Player nameKlaassen
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Squad number4Player namevan Dijk
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Squad number5Player nameAké
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Squad number21Player namede Jong
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Squad number17Player nameBlind
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Squad number2Player nameTimber
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Squad number7Player nameBergwijn
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Squad number22Player nameDumfries
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Squad number20Player nameKoopmeiners
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Squad number10Player nameDepay
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Squad number23Player nameNoppert
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Squad number15Player namede Roon
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Squad number19Player nameWeghorst
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Squad number11Player nameBerghuis
Ecuador
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Squad number7Player nameEstupiñán
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Squad number13Player nameE Valencia
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Squad number23Player nameCaicedo
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Squad number16Player nameSarmiento
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Squad number26Player nameRodríguez
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Squad number19Player namePlata
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Squad number2Player nameTorres
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Squad number20Player nameMéndez
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Squad number3Player nameHincapié
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Squad number17Player namePreciado
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Squad number10Player nameIbarra
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Squad number11Player nameEstrada
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Squad number25Player namePorozo
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Squad number1Player nameGalíndez
Line-ups
Netherlands
Formation 3-4-1-2
- 23Noppert
- 2Timber
- 4van Dijk
- 5Aké
- 22Dumfries
- 20Koopmeiners
Substituted forde Roonat 79'minutes
- 21de Jong
- 17Blind
- 14Klaassen
Substituted forBerghuisat 69'minutes
- 8Gakpo
Substituted forWeghorstat 80'minutes
- 7Bergwijn
Substituted forDepayat 45'minutes
Substitutes
- 1Pasveer
- 3de Ligt
- 6de Vrij
- 9de Jong
- 10Depay
- 11Berghuis
- 12Lang
- 13Bijlow
- 15de Roon
- 16Malacia
- 18Janssen
- 19Weghorst
- 24Taylor
- 25Simons
- 26Frimpong
Ecuador
Formation 3-4-2-1
- 1Galíndez
- 25Porozo
- 2Torres
- 3Hincapié
- 17Preciado
- 20MéndezBooked at 57mins
- 23Caicedo
- 7Estupiñán
- 19Plata
Substituted forIbarraat 90'minutes
- 13E Valencia
Substituted forRodríguezat 90'minutes
- 11Estrada
Substituted forSarmientoat 74'minutes
Substitutes
- 4Arboleda
- 5Cifuentes
- 6Pacho
- 8Gruezo
- 9Preciado
- 10Ibarra
- 12Ramírez
- 14Arreaga
- 15Mena
- 16Sarmiento
- 18Palacios
- 21Franco
- 22Domínguez
- 24Reasco
- 26Rodríguez
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- Referee:
- Mustapha Ghorbal
Match Stats
Live Text
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Match ends, Netherlands 1, Ecuador 1.
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Second Half ends, Netherlands 1, Ecuador 1.
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Corner, Ecuador. Conceded by Daley Blind.
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Attempt saved. Kevin Rodríguez (Ecuador) header from the centre of the box is saved in the bottom left corner. Assisted by Angelo Preciado.
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Attempt blocked. Angelo Preciado (Ecuador) header from outside the box is blocked. Assisted by Jeremy Sarmiento with a headed pass.
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Substitution, Ecuador. Romario Ibarra replaces Gonzalo Plata.
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Substitution, Ecuador. Kevin Rodríguez replaces Enner Valencia because of an injury.
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Nathan Aké (Netherlands) wins a free kick on the left wing.
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Foul by Moisés Caicedo (Ecuador).
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Denzel Dumfries (Netherlands) wins a free kick in the defensive half.
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Foul by Pervis Estupiñán (Ecuador).
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Attempt missed. Angelo Preciado (Ecuador) right footed shot from outside the box is high and wide to the right following a corner.
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Corner, Ecuador. Conceded by Marten de Roon.
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Substitution, Netherlands. Wout Weghorst replaces Cody Gakpo.
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Substitution, Netherlands. Marten de Roon replaces Teun Koopmeiners.
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Foul by Jurriën Timber (Netherlands).
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Jhegson Méndez (Ecuador) wins a free kick in the defensive half.
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Foul by Jurriën Timber (Netherlands).
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Jeremy Sarmiento (Ecuador) wins a free kick on the right wing.
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Foul by Steven Berghuis (Netherlands).

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